Understanding substrate binding and the role of gatekeeping residues in PigC access tunnels

Semi-rational redesign of the substrate binding pocket and access tunnels of prodigiosin ligase PigC enhanced the catalytic efficiency in the synthesis of pyrrolic anti-cancer agents more than 45 times. A molecular understanding was gained on residues V333 and T334 relevant to substrate binding and translocation of small pyrroles through PigC access tunnels.

Automated summary

The semi-rational redesign of substrate binding pocket and access tunnels in PigC significantly enhanced catalytic efficiency in synthesizing anti-cancer agents, while providing a better understanding of residues V333 and T334 related to substrate binding and translocation through PigC access tunnels.