期刊
FOOD RESEARCH INTERNATIONAL
卷 100, 期 -, 页码 514-521出版社
ELSEVIER
DOI: 10.1016/j.foodres.2017.06.040
关键词
Dulse; Water-extract; Anti-inflammatory effect; Phycobiliprotein; Chlorophyll; Pheophorbide
资金
- Knowledge Cluster Initiative (Hakodate Marine Biocluster), MEXT (Ministry of Education, Culture, Sports, Science and Technology, Japan)
The use of dulse (Palmaria palmata) as a source of edible anti-inflammatory products was evaluated in this study. Phycobiliproteins and chlorophyll a were simultaneously extracted from lyophilized dulse leaves via water extraction, and subjected to thermolysin digestion to produce thermolysin-digested water-extract (d-DWE). d-DWE significantly reduced tumor necrosis factor-a, interleukin-6, and nitric oxide in LPS-stimulated murine macrophages (RAW 264.7 cells), and orally administered d-DWE mitigated acute inflammation in carrageenan-induced paw edema of mice. Mass spectrometry revealed d-DWE contained peptide LRDGEIILRY (derived from phycoerythrin beta-chain) and chlorophyll a decomposition products, and they individually reduced the secretion of the proinflammatory mediators in LPS-stimulated RAW 264.7 cells. These results indicate the anti-inflammatory activity could be from a combined effect of phycobiliprotein and chlorophyll a decomposition products prepared from the water-extract of dulse. Thus, inexpensive and safe water-extraction method is effective for the extraction of anti-inflammatory components from dulse.
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