期刊
FOOD & FUNCTION
卷 12, 期 6, 页码 2703-2714出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0fo03353g
关键词
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资金
- Zhejiang Provincial Natural Science Foundation of China [LGF20H060013, LGF21H060011, LGF21H060010]
- Wenzhou Science and Technology Bureau Foundation [ZY2019014, Y2020059, Y20190018]
- Zhejiang Provincial Project for Medical and Health Science and Technology [2020KY190]
- National Natural Science Foundation of China [81871806, 81972094]
Cardamonin (CAR) demonstrates protective effects on rat nucleus pulposus (NP) cells and in an induced rat intervertebral disc degeneration (IVDD) model by inhibiting inflammatory factors, reversing pathological changes, and activating the Nrf2/HO-1 signaling axis, suggesting its potential as a novel therapeutic agent for IVDD.
Intervertebral disc degeneration (IVDD) is one of the major causes of low back pain, but effective therapies are still lacking because of its complicated pathology. It has been demonstrated that increased levels of interleukin-1 beta (IL-1 beta) may promote the development of IVDD. Cardamonin (CAR) is a chalcone extracted from Alpinia katsumadai and other plants. It exhibits an anti-inflammatory effect in multiple diseases. In the present study, we investigated the protective effects of CAR on rat nucleus pulposus (NP) cells under IL-1 beta stimulation in vitro and in a puncture-induced rat IVDD model in vivo. We explored the CAR treatment's inhibition of the expression of inflammatory factors such as cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), nitric oxide (NO), tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) in rat NP cells. Moreover, the up-regulation of matrix metalloproteinase-13 (MMP-13) and thrombospondin motifs 5 (ADAMTS-5) and the degradation of aggrecan and collagen II induced by IL-1 beta were reversed by CAR. Mechanistically, we demonstrated that CAR inhibited nuclear factor kappa B (NF-kappa B) signaling by activating the nuclear factor erythroid-derived 2-like 2 (Nrf2) in IL-1 beta-induced rat NP cells. Furthermore, the protective effect of CAR was shown in the IVDD model through persistent intragastric administration. Taken together, our results revealed that CAR could activate the Nrf2/HO-1 signaling axis and be a novel agent for IVDD therapy.
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