Measuring anion transport selectivity: a cautionary tale

pH-dependent liposomal assays are often used to determine anion selectivity in transmembrane anion transport experiments. We discuss the validity and limitations of these assays, and provide guidelines for their use to avoid misleading results.

Automated summary

pH-dependent liposomal assays are commonly used to determine anion selectivity in transmembrane anion transport experiments. There are both validity and limitations to consider when using these assays, and guidelines are provided to ensure accurate results.