期刊
FOOD CONTROL
卷 78, 期 -, 页码 1-6出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2017.02.027
关键词
direct pentaplex PCR; Species identification; Commercial jerky products; Authenticity; 18S rRNA
资金
- Ministry of Food and Drug Safety in Korea [16163MFDS003]
- Ministry of Food & Drug Safety (MFDS), Republic of Korea [DY0002256294-16163실용연003-1] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The direct pentaplex PCR assay was developed for simultaneous identification using species-specific primer sets and a universal eukaryotic primer set in processed jerky products without DNA extraction. The specific primer sets of target meat species amplified the expected 83-, 133-, 166-, and 204-bp PCR products for pork, chicken, beef, and duck, respectively, and obtained no cross-reactivity against a total of sixteen animal species. A universal eukaryotic primer set amplified a 99-bp conserved fragment in all meat species. To evaluate the sensitivity of this assay, the different percentages of jerky samples were prepared with the meat species having the possibility to be mixed. Adulterated beef jerky samples contaminated with 0.1, 0.5, 1, 5, 10, and 50% pork and adulterated duck jerky samples contaminated with 0.1, 0.5, 1, 5, 10, and 50% chicken were prepared in a laboratory. The detection level of direct pentaplex PCR was below 0.1% pork in adulterated beef jerky and 0.1% chicken in adulterated duck jerky. The optimized assay was also applied to the analysis of commercial food and feed jerky products. The meat species in commercial jerky products were successfully identified without DNA extraction. (C) 2017 Elsevier Ltd. All rights reserved.
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