Effects of Quillaja saponaria extract and Nα-lauroyl-L-arginine ethyl ester on reducing selected foodborne pathogens in vitro and maintaining quality of fresh-cut endive (Cichorium endivia L.) at pilot plant scale

The aim of the present study was to evaluate the applicability of Quillaja saponaria extract (QSE) and N-alpha-lauroyl-L-arginine ethyl ester (LAE) as antimicrobial wash water additives in fresh-cut lettuce processing. Antibacterial activities of LAE and QSE against selected strains of the foodborne pathogens Salmonella enterica, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and Listeria monocytogenes were examined in vitro. Minimum inhibitory concentrations determined by broth microdilution assay demonstrated that LAE exhibited a strong antimicrobial activity with MICs between 4 and 32 mu g/mL against all tested strains, whereas QSE showed a weaker antimicrobial activity with MICs >512 mu g/mL. On a pilot-plant scale, the effects of warm water washing at 45 degrees C for 120 s with and without 40 mg/L QSE or 100 mg/L LAE as well as cold water washing at 4 degrees C for 120 s with QSE or LAE, respectively, of shredded endive (Cichorium endivia L.) were investigated regarding microbiological and sensory quality as well as physiological properties. Samples were analyzed for headspace O-2 and CO2 levels, phenylalanine-ammonia-lyase activity and contents of nitrite and nitrate during nine days of cold storage at 4 degrees C. By analogy to its antimicrobial effect against the foodborne pathogens in vitro, LAE allowed up to 4 log(10) cfu/mL reduction of the microbial load in the washing water of the pilot plant, and might therefore reduce cross-contamination while saving water. The addition of LAE to warm washing water impaired sensory properties of fresh-cut endive during storage, which was predicted by chlorophyll fluorescence imaging analyses. QSE treatment combined with warm water washing best retained sensory appearance throughout our study, being possibly suitable for the production of premium products. (C) 2016 Elsevier Ltd. All rights reserved.