期刊
RSC ADVANCES
卷 11, 期 15, 页码 8516-8520出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ra09512e
关键词
-
资金
- National Natural Science Foundation of China General Program [81671745]
A novel turn-on fluorescent probe NTR-NO2 was synthesized for detecting NTR with high efficiency, sensitivity, and selectivity, which can be used for diagnosing tumor hypoxia and detecting endogenous NTR in living cells.
Nitroreductase (NTR) may be more active under the environment of hypoxic conditions, which are the distinctive features of the multiphase solid tumor. It is of great significance to effectively detect and monitor NTR in the living cells for the diagnosis of hypoxia in a tumor. Here, we synthesized a novel turn-on fluorescent probe NTR-NO2 based on a fused four-ring quinoxaline skeleton for NTR detection. The highly efficient probe can be easily synthesized. The probe NTR-NO2 showed satisfactory sensitivity and selectivity to NTR. Upon incubation with NTR, NTR-NO2 could successively undergo a nitro reduction reaction and then generate NTR-NH2 along with significant fluorescence enhancement (30 folds). Moreover, the fluorescent dye NTR-NH2 exhibits a large Stokes shift (Delta lambda = 111 nm) due to the intramolecular charge transfer (ICT) process. As a result, NTR-NO2 displayed a wide linear range (0-4.5 mu g mL(-1)) and low detection limit (LOD = 58 ng mL(-1)) after responding to NTR. In addition, this probe was adopted for the detection of endogenous NTR within hypoxic HeLa cells.
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