期刊
FOOD CHEMISTRY
卷 227, 期 -, 页码 245-254出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.01.100
关键词
STEC; Orange pectic oligosaccharides; Anti-adhesion; Shiga toxin 2; TaqMan RT-qPCR; rRNA depurination; HT29 cells
Shiga toxin (Stx)-producing, food-contaminating Escherichia coil (STEC) is a major health concern. Plant derived pectin and pectic-oligosaccharides (POS) have been considered as prebiotics and for the protection of humans from Stx. Of five structurally different citrus pectic samples, POS1, POS2 and modified citrus pectin 1 (MCP1) were bifidogenic with similar fermentabilities in human faecal cultures and arabinose-rich POS2 had the greatest prebiotic potential. Pectic oligosaccharides also enhanced lactobacilli growth during mixed batch faecal fermentation. We demonstrated that all pectic substrates were anti-adhesive for E. coil O157:H7 binding to human HT29 cells. Lower molecular weight and deesterification enhanced the anti-adhesive activity. We showed that all pectic samples reduced Stx2 cytotoxicity in HT29 cells, as measured by the reduction of human rRNA depurination detected by our novel TagManbased RT-qPCR assay, with POS1 performing the best. POS1 competes with Stx2 binding to the Gb3 receptor based on ELISA results, underlining the POS anti-STEC properties. (C) 2017 Elsevier Ltd. All rights reserved.
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