期刊
FOOD CHEMISTRY
卷 217, 期 -, 页码 628-636出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.09.021
关键词
Clenbuterol; Molecular imprinted monolithic columns; Two-dimensional liquid chromatography; Pork liver; Swine urine
资金
- National Natural Science Foundations of China [81573391, 81173024]
- National Key Projects of China [81227802]
- China Postdoctoral Science Foundation [2014M562428]
- Shannxi Province Health Department Scientific Foundation [2014D73]
A novel method coupling molecular imprinted monolithic column with two-dimensional liquid chromatography was developed and validated for the analysis of clenbuterol in pork liver and swine urine samples. The polymers were characterized by using Fourier transform infrared spectroscopy, nitrogen adsorption desorption analyses, frontal analysis and the adsorption of selectivity. The results indicated that the imprinted columns were well prepared and possessed high selectivity adsorption capacity. Subsequently, the MIMC-2D-LC (molecular imprinted monolithic column-two dimensional liquid chromatography) method was developed for the selective analysis of clenbuterol in practical samples. The accuracy ranged from 94.3% to 99.7% and from 93.7% to 99.6% for liver and urine, respectively. The relative standard deviation (RSD) of repeatability was lower than 8.6% for both analyses. The limit of detections was 16 ng.mL(-1) for liver and 25 ng.mL(-1) for urine, respectively. Compared with the reported methods, the disturbance of endogenous impurity could be avoided by the 2D-LC method. (C) 2016 Elsevier Ltd. All rights reserved.
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