期刊
FOOD & FUNCTION
卷 12, 期 11, 页码 4783-4796出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1fo00157d
关键词
-
资金
- National Natural Science Foundation of China [82003447, 32001770]
- Natural Science Foundation of Shandong Province [ZR2019PC014]
- China Postdoctoral Science Foundation [2019M652327]
- Young Scholars Program of Shandong University [2018WLJH34]
- Laboratory for Marine Drugs and Bioproducts of the Qingdao National Laboratory for Marine Science and Technology [LMDBKF-2019-05]
The study showed that DHA-PL and EPA-PL inhibited TNF-alpha-induced lipolysis, increased DHA and EPA content in adipocytes, and regulated metabolic pathways by activating the SIRT1 pathways. Additionally, they improved glucose metabolism by enhancing glucose uptake and transporter translocation.
Some chronic diseases such as cancer-associated cachexia (CAC) and obesity are associated with the overproduction of tumor necrosis factor-alpha (TNF-alpha) that stimulates excess lipolysis in adipocytes. Our previous studies have shown that docosahexaenoic acid-enriched phospholipids (DHA-PL) and eicosapentaenoic acid-enriched phospholipids (EPA-PL) ameliorated CAC and obesity-related metabolic disorders. To identify the molecular mechanisms involved, we examined the impact and the associated signaling pathways of DHA-PL and EPA-PL on TNF-alpha-induced lipolysis in 3T3-L1 adipocytes. The present results revealed that DHA-PL and EPA-PL inhibited the TNF-alpha-induced increase of glycerol release and protected lipid droplets. In addition, DHA-PL and EPA-PL increased DHA and EPA contents in the phospholipid fraction of adipocytes, respectively. Moreover, DHA-PL and EPA-PL enhanced sirtuin 1 (SIRT1) deacetylase activity and its protein expression. By activating SIRT1, DHA-PL and EPA-PL upregulated the G0/G1 switch gene 2 protein level to inhibit adipose triglyceride lipase activity, activate AMP-activated protein kinase to reverse the downregulation of perilipin expression and phosphorylation of hormone-sensitive lipase (HSL) at Ser565 and prevent the phosphorylation of HSL at Ser660. Furthermore, DHA-PL and EPA-PL improved glucose uptake and glucose transporter type 4 translocation to the plasma membrane in TNF-alpha-treated adipocytes. Thus, it was concluded that DHA-PL and EPA-PL inhibit TNF-alpha-induced lipolysis in 3T3-L1 adipocytes by activating the SIRT1 pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据