期刊
FOOD CHEMISTRY
卷 221, 期 -, 页码 1747-1753出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.10.110
关键词
Processed food; Multi-allergen analysis; On-line SPE; SRM; Mass spectrometry; Cookies
资金
- project Safe & Smart- Nuove tecnologie abilitanti per la food safety e l'integrita delle filiere agro-alimentari in uno scenario globale - National CL.AN-Cluster agroalimentare nazionale programma area 2
Allergenic ingredients in pre-packaged foods are regulated by EU legislation mandating their inclusion on labels. In order to protect allergic consumers, sensitive analytical methods are required for detect allergen traces in different food products. As a follow-up to our previous investigations, an optimized, sensitive, label-free LC-MS/MS method for multiplex detection of five allergenic ingredients in a processed food matrix is proposed. A cookie base was chosen as a complex food matrix and home-made cookies incurred with whole egg, skimmed milk, soy flour, ground hazelnut and ground peanut were prepared at laboratory scale. In order to improve the analytical workflow both protein extraction and purification protocols were optimized and finally a sensitive streamlined SRM based analytical method for allergens detection in incurred cookies was devised. The effect of baking on the detection of selected markers was also investigated. (C) 2016 Elsevier Ltd. All rights reserved.
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