期刊
FOOD CHEMISTRY
卷 221, 期 -, 页码 855-863出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.11.086
关键词
Crocus sativus L.; hCA inhibitors; Microwave-assisted extraction; NIR spectroscopy; Geographical origin; HPLC-DAD; Principal component analysis
资金
- e-ALIERB Project [LR13/2008]
A microwave-assisted extraction method was optimised for the recovery of bioactive compounds from Crocus sativus L. stigmas with the use of water/ethanol mixture. HPLC-DAD was employed to evaluate the extraction parameters, in particular, solvent type and volume, and the duration of the procedure. Microwave-assisted extraction enhanced the recovery of the active principles, limiting extraction time and solvent waste. Moreover, NIR experiments were performed in order to compare spectra in pseudo-absorbance of Saffron samples with different geographical origins through the application of the chemometric techniques. Moreover, the biological evaluation of crocin 1, safranal and its semisynthetic derivatives as selective inhibitors of five isoforms of human carbonic anhydrase was also explored. (C) 2016 Elsevier Ltd. All rights reserved.
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