A new 7-diethylamino-4-hydroxycoumarin based reversible colorimetric/fluorometric probe for sequential detection of Al3+/PPi and its potential use in biodetection and bioimaging applications

Herein, we have developed a new 7-diethylamino-4-hydroxycoumarin appended acylhydrazone (L) probe using a simple condensation reaction. The structure of the probe L was characterized via single crystal X-ray analysis. This probe L can selectively detect Al3+ metal ions via a colorimetric as well as fluorescence turn-on strategy over other metal ions. Besides, the probe can also detect the aluminium ions in pharmaceutical tablets. The resulting L-Al3+ ensemble has been exploited as a turn-off fluorescence sensor for the detection of pyrophosphate (PPi) anion through metal displacement or by using a decomplexation approach. Based on reversible off-on-off emission characteristics, the probe was applied for the construction of molecular logic gates and the development of a paper strip kit for on-site detection applications. In addition, this ensemble can also be utilized to detect PPi released during DNA synthesis and biological extracts, and in live cells.

Automated summary

The newly developed 7-diethylamino-4-hydroxycoumarin appended acylhydrazone probe can selectively detect Al3+ metal ions and aluminum ions in pharmaceutical tablets. Moreover, it can be used for detecting pyrophosphate (PPi) anions and has applications in constructing molecular logic gates and on-site detection kits.