期刊
FOOD CHEMISTRY
卷 230, 期 -, 页码 547-552出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.03.081
关键词
Tuneable surface enhanced Raman spectroscopy; Thin-layer chromatography; Histamine
资金
- collaborative innovation center of food safety and quality control in Jiangsu Province
- Jiangnan University Fundamental Research Funds for the Central Universities [JUSRP116035]
- National Key Research and Development program [2016YFD0400304]
- National Natural Science Foundation of China [31471584]
Reliable screening of histamine in fish was of urgent importance for food safety. This work presented a highly selective surface enhanced Raman spectroscopy (SERS) method mediated by thin-layer chromatography (TLC), which was tailored for identification and quantitation of histamine. Following separation and derivatization with fluram, plates were assayed with SERS, jointly using silver nanoparticle and NaCl. The latter dramatically suppressed the masking effect caused by excessive fluram throughout the plate, thus offering clear baseline and intensive Raman fingerprints specific to the analyte. Under optimized conditions, the usability of this method was validated by identifying the structural fingerprints of both targeted and unknown compounds in fish samples. Meanwhile, the quantitative results of this method agreed with those by an HPLC method officially suggested by EU for histamine determination. Showing remarkable cost-efficiency and user-friendliness, this facile TLC-SERS method was indeed screening-oriented and may be more attractive to controlling laboratories of limited resource. (C) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据