期刊
FOOD CHEMISTRY
卷 218, 期 -, 页码 463-470出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.07.090
关键词
2R,3R-Dihydromyricetin; Staphylococcus aureus; Antibacterial activity; Membrane damage; DNA binding
资金
- programme for new century excellent talents in univ. [NCET-10-0591]
- Sichuan Youth Sci-tech Fund [2012JQ0019]
- National Nature Science Fundation of China [31571936]
The antibacterial activity and mechanism of 2R,3R-dihydromyricetin (DMY) against Staphylococcus aureus were investigated. The minimum inhibitory concentration of DMY against S. aureus was 0.125 mg/ml, and the growth inhibitory assay also revealed that DMY showed a potent antibacterial activity against S. aureus. Massive nucleotide leakage and flow cytometric analysis demonstrated that DMY disrupted the membrane integrity of S. aureus. Morphological changes and membrane hyperpolarization of S. aureus cells treated with DMY further suggested that DMY destroyed cell membrane. Meanwhile, DMY probably interacted with membrane lipids and proteins, causing a significant reduction in membrane fluidity and changes in conformation of membrane protein. Moreover, DMY could interact with S. aureus DNA through the groove binding mode. Overall, the results suggested that DMY could be applied as a candidate for the development of new food preservatives as it achieved bactericidal activity by damaging cell membrane and binding to intracellular DNA. (C) 2016 Published by Elsevier Ltd.
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