期刊
FEMS MICROBIOLOGY REVIEWS
卷 45, 期 2, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsre/fuab008
关键词
acetogen; gas fermentation; genetic engineering; metabolic engineering; biotechnology; fuels and chemicals
类别
资金
- Loughborough University departmental faculty start-up funds
- Loughborough University
- BBSRC [BB/L013940/1] Funding Source: UKRI
The continuous mining and use of petroleum resources have harmful environmental impacts, leading to the search for sustainable alternatives for chemicals and fuels production. Bioprocesses utilizing microorganisms have gained interest as a potential solution.
Unabated mining and utilisation of petroleum and petroleum resources and their conversion to essential fuels and chemicals have drastic environmental consequences, contributing to global warming and climate change. In addition, fossil fuels are finite resources, with a fast-approaching shortage. Accordingly, research efforts are increasingly focusing on developing sustainable alternatives for chemicals and fuels production. In this context, bioprocesses, relying on microorganisms, have gained particular interest. For example, acetogens use the Wood-Ljungdahl pathway to grow on single carbon C1-gases (CO2 and CO) as their sole carbon source and produce valuable products such as acetate or ethanol. These autotrophs can, therefore, be exploited for large-scale fermentation processes to produce industrially relevant chemicals from abundant greenhouse gases. In addition, genetic tools have recently been developed to improve these chassis organisms through synthetic biology approaches. This review will focus on the challenges of genetically and metabolically modifying acetogens. It will first discuss the physical and biochemical obstacles complicating successful DNA transfer in these organisms. Current genetic tools developed for several acetogens, crucial for strain engineering to consolidate and expand their catalogue of products, will then be described. Recent tool applications for metabolic engineering purposes to allow redirection of metabolic fluxes or production of non-native compounds will lastly be covered.
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