期刊
FOOD CHEMISTRY
卷 222, 期 -, 页码 105-112出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.12.015
关键词
Glucansucrase; Lactobacillus reuteri SK24.003; Enzymological property; Glucan biosynthesis; Structure
资金
- National Natural Science Foundation of China [31000764, 31230057]
- International Cooperative Program of Jiangsu Province [BZ2012031]
- Science & Technology Pillar Program of Jiangsu Province [BE2013647, BE2014703]
- Fundamental Research Funds for the Central Universities [JUSRP51616B]
Glucansucrase was obtained from Lactobacillus reuteri SK24.003 and its characterizations and in vitro biosynthesis for glucose polymer and oligosaccharides were investigated. The final specific activity of glucansucrase was 1.3 IU/mg protein with 8.6-fold purification and 8.7% recovery. The molecular weight of purified enzyme was 166.0 kDa. The glucansucrase exhibited optimum activity at 30-35 degrees C, whereas the maximum activity was obtained at pH 5.0-5.5. The double-charged ions including Mg2+, Mn2+, Ni2+, Co2+, Ca2+, Fe2+ and Zn2+ activated the glucansucrase activities and Ca2+ ion highly stimulated the activity by approximately 4 times. K-m, V-max and k(cat) of purified glucansucrase were calculated to be 3.7 mM, 0.8 IU/mg and 18.2 1/s, respectively. For in vitro biosynthesis, glucansucrase synthesized 1,6-,1,4-alpha-D-glucan with a molecular weight of 2.5 x 10(7) g/mol from sucrose as initial primer. Moreover, maltose acceptor-products synthesized by glucansucrase were composed of panose, maltotriose, maltotetraose, tetrasaccharide and pentasaccharide products analogues with an alpha-1,4/alpha-1,6 alternating structure. (C) 2016 Elsevier Ltd. All rights reserved.
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