期刊
FOOD CHEMISTRY
卷 221, 期 -, 页码 91-99出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.10.073
关键词
Pectin; Microwave-assisted extraction; Surface Response Methodology; HPSEC; FTIR; Light scattering
资金
- Algerian Ministry of Higher Education and Scientific Research
- IBF-CNR of Palermo (Italy)
Optimization of microwave-assisted extraction (MAE) of water-soluble pectin (WSP) from Opuntia ficus indica cladodes was performed using Response Surface Methodology. The effect of extraction time (X-1), microwave power (X-2), pH (X-3) and solid-to-liquid ratio (X-4) on the extraction yield was examined. The optimum conditions of MAE were as follows: X-1 = 2.15 min; X-2 = 517 W; X-3 = 2.26 and X-4 = 2g/30.6 mL. The maximum obtained yield of pectin extraction was 12.57%. Total carbohydrate content of WSP is about 95.5% including 34.4% of Galacturonic acid. Pectin-related proteins represent only the 0.66% of WSP mass. HPSEC and light scattering analyses reveal that WSP is mostly constituted of high molecular pectin and FTIR measurements show that the microwave treatment does not alter the chemical structure of WSP, in which Galacturonic acid content and yield are 34.4% and 4.33%, respectively. Overall, application of MAE can give rise to high quality pectin. (C) 2016 Elsevier Ltd. All rights reserved.
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