期刊
FOOD CHEMISTRY
卷 216, 期 -, 页码 80-86出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.07.185
关键词
Vitis vinifera L.; Must; Wine; HRM; Authenticity
资金
- Portuguese Foundation for Science and Technology (FCT) through the project Biosensor Development for Wine Traceability in the Douro Region - WineBioCode [P TDC/AGR-ALI/117341/2010-FCOMP-01-0124-FEDER-019439, SFRH/BD/44781/2008, SFRH/BPD/70378/2010]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/44781/2008] Funding Source: FCT
Wine authenticity methods are in increasing demand mainly in Denomination of Origin designations. The DNA-based methodologies are a reliable means of tracking food/wine varietal composition. The main aim of this work was the study of High Resolution Melting (HRM) application as a screening method for must and wine authenticity. Three sample types (leaf, must and wine) were used to validate the three developed HRM assays (Vv1-705 bp; Vv2-375 bp; and. Vv3-119 bp). The Vv1 HRM assay was only successful when applied to leaf and must samples. The Vv2 HRM assay successfully amplified all sample types, allowing genotype discrimination based on melting temperature values. The smallest amplicon, Vv3, produced a coincident melting curve shape in all sample types (leaf and wine) with corresponding genotypes. This study presents sensitive, rapid and efficient HRM assays applied for the first time to wine samples suitable for wine authenticity purposes. (C) 2016 Elsevier Ltd. All rights reserved.
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