期刊
NATURE REVIEWS METHODS PRIMERS
卷 1, 期 1, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s43586-021-00029-y
关键词
-
资金
- BBSRC iCASE award
- Astra Zeneca
- Knut and Alice Wallenberg Foundation [2016.0204]
- Swedish Research Council [2017-05327]
- Science for Life (SciLifeLab)
- National Microscopy Infrastructure [VR-RFI 2019-00217]
- European Proteomics Infrastructure Consortium EPIC-XS [823839]
- EU Horizon 2020 programme
- Canadian Institutes of Health Research [FDN143301]
- KRESCENT Post-Doctoral Fellowship
- Canadian Institutes of Health Research Fellowship
- Deutsche Forschungsgemeinschaft [403222702/SFB 1381, FOR 1905, FOR 2743]
- Germany's Excellence Strategy [CIBSS - EXC-2189, 390939984]
- European Research Council Consolidator Grant [648235]
- European Union Marie Curie Initial Training Networks program PerICo [812968]
- Genome Canada/Genome British Columbia [264PRO]
- National Institute of General Medical Sciences [GM114141, T32GM007388]
- National Institute of Child Health and Human Development [HD089275]
- Edward Mallinckrodt Jr. foundation
- Swedish Cancer Society
- Erling-Persson Family Foundation
- Swedish Childhood Cancer Foundation
- Swedish Foundation for Strategic Research
- Swedish Research Council
- EU
- National Institutes of Health (NIH) [1UL1TR001430, R01AG064932, R01AG061706, R01DK110520]
- European Research Council (ERC) [648235] Funding Source: European Research Council (ERC)
- Marie Curie Actions (MSCA) [812968] Funding Source: Marie Curie Actions (MSCA)
Proteins in eukaryotic cells play crucial roles in different subcellular niches, with dynamic movements essential for cellular processes. Investigating protein localization and relocalization is important for understanding their functions and associated pathologies.
The eukaryotic cell is compartmentalized into subcellular niches, including membrane-bound and membrane-less organelles. Proteins localize to these niches to fulfil their function, enabling discreet biological processes to occur in synchrony. Dynamic movement of proteins between niches is essential for cellular processes such as signalling, growth, proliferation, motility and programmed cell death, and mutations causing aberrant protein localization are associated with a wide range of diseases. Determining the location of proteins in different cell states and cell types and how proteins relocalize following perturbation is important for understanding their functions, related cellular processes and pathologies associated with their mislocalization. In this Primer, we cover the major spatial proteomics methods for determining the location, distribution and abundance of proteins within subcellular structures. These technologies include fluorescent imaging, protein proximity labelling, organelle purification and cell-wide biochemical fractionation. We describe their workflows, data outputs and applications in exploring different cell biological scenarios, and discuss their main limitations. Finally, we describe emerging technologies and identify areas that require technological innovation to allow better characterization of the spatial proteome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据