期刊
FOOD CHEMISTRY
卷 230, 期 -, 页码 125-134出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.03.025
关键词
Nigella species; RP-HPLC fractionation; Protein fractions; Molecular; Angiotensin converting enzyme; Fractionation; Antioxidant activity
资金
- Jordanian Ministry of Higher Education and Scientific Research/Scientific Research Support Fund
- Jordan University of Science and Technology
This study aimed to investigate the molecular and bio-functional properties of protein fractions from Nigella damascena and Nigella arvensis, including the albumin, globulin, glutein-1, glutein-2 and prolamin fractions. Protein subunits were not observed in globulin and prolamin fractions. No peaks appeared in RP-HPLC chromatograms of globulin for either species. Two predominant peaks were observed in the RP-HPLC profiles of all protein fractions. Proteins separated by RP-HPLC have potential inhibitory and antioxidant activities in all fractions. Optimum ACE-inhibitory and antioxidant activities of proteins separated by RP-HPLC were observed in glutein-2 and albumin, respectively, for both species. For pepsin and combined pepsin-trypsin hydrolyses, the highest degree of hydrolysis (DH) was obtained in glutein-2 fraction of Nigella arvensis. Highest ACE-inhibitory activity of hydrolyzed protein fractions was found at 4 h via pepsin hydrolysis in globulin fraction of Nigella damascena. Highest antioxidant activities of hydrolyzed protein fractions were found in glutelin-2 for both species. (C) 2017 Elsevier Ltd. All rights reserved.
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