期刊
FOOD CHEMISTRY
卷 229, 期 -, 页码 396-402出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.02.088
关键词
DPO primers; High-throughput; GMO screening; Wide coverage; Capillary electrophoresis
资金
- National Science and Technology Major Project [2016ZX08012-001]
- Basic Scientific Research Foundation in the Chinese Academy of Inspection and Quarantine [2015JK005]
- Science and Technology Planning Project of Guangzhou [2014J4100105]
- Science and Technology Planning Project of Guangdong Province [2014A040401029]
High throughput screening systems are the preferred solution to meet the urgent requirement of increasing number of genetically modified organisms (GMOs). In this study, we have successfully developed a multiplex GMO element screening system with dual priming oligonucleotide (DPO) primers. This system can detect the cauliflower mosaic virus 35S (CaMV 35S), terminator of nopaline synthase gene (NOS), figwort mosaic virus 35S (FMV 35S) promoter, neomycin phosphotransferaseII (NPTII), Bt Cry 1Ab, phosphinothricin acetyltransferase genes (bar) and Streptomyces viridochromogenes (pat) simultaneously, which covers more than 90% of all authorized GMO species worldwide. This system exhibits a high tolerance to annealing temperatures, high specificity and a limit of detection equal to conventional PCR. A total of 214 samples from markets, national entry-exit agencies, the Institute for Reference Materials and Measurement (IRMM) and the American Oil Chemists' Society (AOCS) were also tested for applicability. This screening system is therefore suitable for GMO screening. (C) 2017 Elsevier Ltd. All rights reserved.
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