期刊
ANALYST
卷 146, 期 7, 页码 2292-2300出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0an02069a
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资金
- JSPS KAKENHI [20K05575]
- Grants-in-Aid for Scientific Research [20K05575] Funding Source: KAKEN
The study focused on the impact of liquid chromatography-tandem mass spectrometry interfaced with electrospray ionization on tryptophan-derived metabolites, revealing fragmentation during ESI. The quantitative analysis used multiple reaction monitoring technology and proposed a method for high sensitivity analysis.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is interfaced with electrospray ionization (ESI), which generally produces intact gas-phase ions of biomolecules. However, ESI induces the fragmentation of tryptophan-derived metabolites, which are known to act as neurotransmitters and psychoactive drugs. Tryptophan-derived metabolites undergo N-C-alpha bond dissociation during ESI, producing a fragment ion with a spiro[cyclopropane-indolium] backbone. Fragmentation is suppressed by the presence of an alpha-carboxyl group and the modification of amino groups. In particular, tryptamine and serotonin, which lack such functional groups, produce more intense fragment-ion signals than protonated molecules. The multiple reaction monitoring (MRM)-based quantitative analysis of tryptamine and serotonin used the fragment ions produced from in-source collision-induced dissociation as the precursor ions, which improved the signal-to-noise ratio of the resulting spectra. The present method allows for the quantitative analysis of tryptamine and serotonin with high sensitivity.
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