期刊
ANALYTICAL METHODS
卷 13, 期 18, 页码 2092-2098出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ay00209k
关键词
-
资金
- National Natural Science Foundation of China [21974101, 21675119]
- Large-scale Instrument and Equipment Sharing Foundation of Wuhan University
This study presents an improved MCH/DNA-Au-QD sensor with increased signal to background ratio, outperforming the original sensor and introducing a new approach for performance optimization.
Nanosurface energy transfer (NSET)-based sensors have been widely developed using various pairs of nanomaterials including gold nanoparticles (AuNPs) and quantum dots (QDs). However, a low signal to background ratio is one of the most important problems that researchers are continually trying to solve. Herein, we present a 6-mercaptohexanol (MCH) modified MCH/DNA-Au-QD sensor for the detection of nucleic acids and MUC1. Interestingly, an unexpected effect of MCH was found in enhancing the fluorescence recovery ratio, therefore yielding a higher signal to background ratio. Through further investigation, we perceive the enhancement as a result of lowering of the NSET efficiency between free DNA-AuNPs and free DNA-QDs, which arises from the stretching of adsorbed DNA on the surface of AuNPs. The employment of MCH endowed the sensor with a wider linear range from 5 nM to 120 nM and a relatively lower LOD of 1.19 nM in nucleic acid detection, outperforming the original DNA-Au-QD sensor. Furthermore, the application of the sensor can be further extended to MUC1 detection. This study offers a better understanding of the NSET process between QDs and AuNPs and also initiates a new approach for the performance optimization of analogous NSET-based sensors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据