Modulation of chemical stability and in vitro bioaccessibility of beta-carotene loaded in kappa-carrageenan oil-in-gel emulsions

In the present paper, ionotropically structured kappa-carrageenan based oil-in-gel (o/g) emulsions were tested as potential carrier systems for the delivery of beta-carotene. In situ ionic gelation was induced by Na+, K+ or Ca2+ added at the level of 0.2-0.6% (w/w). All o/g emulsions exerted a true gel like behaviour with storage modulus (G') being reduced according to the order: K+>Ca2+>Na+. Ionic gelation induced a moderate increase in the microscopically assessed lipid droplets radii. O/g emulsions containing monovalent ions exerted the highest beta-carotene retention throughout isothermal storage particularly at high (37 and 55 degrees C) temperatures. Notwithstanding, increasing ionic strength resulted in acceleration of b-carotene degradation rates for all cation species. beta-Carotene bioaccessibility was significantly lower in Ca2+ o/g emulsions due to the formation of complexes between the biopolymer matrix containing b-carotene and bile salts. A good correlation between b-carotene bioaccessibility, physical and colloidal aspects of the micellar digesta fractions was observed. (C) 2016 Elsevier Ltd. All rights reserved.