Proteomic profiling of camel and cow milk proteins under heat treatment

Cow and camel milk proteins before and after heat treatment at 80 degrees C for 60 min were identified using LC/MS and LC-MS/MS following monodimensional electrophoresis. The database used for the identification of camel and cow proteins was set from http://www.uniprot.org/. The obtained results showed that, after heating, camel milk at 80 degrees C for 60 min, camel alpha-lactalbumin (alpha-la) and peptidoglycan recognition protein (PGRP) were not detected while camel serum albumin (CSA) was significantly diminished. When heating cow milk at 80 degrees C for 60 min, alpha-lactalbumin (alpha-la) and beta-lactoglobulin (beta-lg) were not significantly detected. Moreover, 19 protein bands from SDS-PAGE were analyzed and a total of 45 different proteins were identified by LC-MS/MS. Casein fractions were kept intact under a heat treatment of 80 degrees C during 60 min of both camel and cow milks. Camel and bovine whey proteins were affected by a heat treatment of 80 degrees C for 60 min. (C) 2016 Elsevier Ltd. All rights reserved.