期刊
FOOD CHEMISTRY
卷 237, 期 -, 页码 290-296出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.05.115
关键词
3-Methyl-quinoxaline-2-carboxylic acid; Immunoaffinity column purification; High-performance liquid chromatography with ultraviolet detection; Colloidal gold-based immunochromatographic assay; Edible tissues
资金
- Fundamental Research Funds for the Central Universities [2662017PY049]
- Risk Assessment of Unknown and known hazard factors of livestock and poultry products [GJFP2017007]
- Special Fund for Agro-scientific Research in the Public Interest [201203040]
- Ministry of Agriculture [2014-S12]
An immunoaffinity column (IAC) for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) from porcine muscle and the liver as well as the methods for its determination by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and a colloidal gold-based immunochromatographic assay (GICA) were developed. This is the first study that describes a clean-up method that employs an IAC for the determination of MQCA. Using the HPLC-UV method, the limits of detection and limits of quantification for MQCA in different samples were 1.0-3.0 mu g kg(-1) and 4.0-10.0 mu g kg(-1), respectively. The average recoveries of MQCA that was spiked into samples at concentrations of 4.0-100 mu g kg(-1) were 80.1-87.7%, with relative standard deviations of less than 8.5%. Using the GICA method, the limits of detection for MQCA in different samples were 10 mu g kg(-1) and 50 mu g kg(-1). No false negatives or false positives were observed. (C) 2017 Elsevier Ltd. All rights reserved.
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