期刊
FOOD CHEMISTRY
卷 218, 期 -, 页码 152-158出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.09.058
关键词
Response surface methodology; Flavonoids; Moringa oleifera leaf; Subcritical ethanol extraction; Antioxidant property
资金
- National Natural Science Foundation of China [21376166]
A large-scale process to extract flavonoids from Moringa oleifera leaf by subcritical ethanol was developed and HPLC-MS analysis was conducted to qualitatively identify the compounds in the extracts. To optimize the effects of process parameters on the yield of flavonoids, a Box-Behnken design combined with response surface methodology was conducted in the present work. The results indicated that the highest extraction yield of flavonoids by subcritical ethanol extraction could reach 2.60% using 70% ethanol at 126.6 degrees C for 2.05 h extraction. Under the optimized conditions, flavonoids yield was substantially improved by 26.7% compared with the traditional ethanol reflux method while the extraction time was only 2 h, and obvious energy saving was observed. FRAP and DPPH' assays showed that the extracts had strong antioxidant and free radical scavenging activities. (C) 2016 Elsevier Ltd. All rights reserved.
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