期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 102, 期 -, 页码 93-101出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2017.01.021
关键词
Silymarin; Acrylamide; Nrf2; Antioxidant; Oxidative stress
资金
- Natural Science Foundation of Heilongjiang Province [590024]
- National Science and Technology Program [2013M102208]
Silymarin (SM) is a well-known antioxidant, anti-inflammatory and anti-cancer compound extracted from the milk thistle. Here, we investigated the protective effect of SM against acrylamide (AA)-induced neurotoxicity, mainly caused by oxidative stress, via activation of the nuclear transcription factor E2 related factor 2 (Nrf2) signalling pathway in P02 cells. The mrr reduction assay was used to measure cell viability in various drug-treated groups and demonstrated that SM could increase cell viability in AA treated PC12 cells. We then measured the reactive oxygen species (ROS) levels by the peroxide-sensitive fluorescent probe DCFH-DA and intracellular glutathione (GSH) and malondialdehyde (MDA) levels by absorption spectrdphotometry. Our data revealed that SM could reduce ROS and MDA levels and increase GSH levels in AA-induced PC12 cells. To identify a potential mechanism for SM-induced protection, we measured the mRNA and protein expression levels of Nrf2 and its downstream target antioxidants glutathione peroxidase (Gpx), glutamate cysteine ligase catalytic subunit (GCLC) and glutamate cysteine ligase modifier subunit (GCLM) by quantitative real-time PCR and Western blot, respectively. The results suggested that SM could activate Nrf2 signalling and increase the expression of Nrf2, Gpx, GCLC and GCLM in AA-treated P02 cells. In conclusion, SM can effectively alleviate AA-induced neurotoxicity in PC12 cells. (C) 2017 Elsevier Ltd. All rights reserved.
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