期刊
CHEMICAL COMMUNICATIONS
卷 57, 期 27, 页码 3367-3370出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc00325a
关键词
-
资金
- Natural Science Foundation of China [21974018, 22074011, 21727811]
- Fundamental Research Funds for the Central Universities [N2005015, N2005027]
- Liaoning Revitalization Talents Program [XLYC1907191, XLYC1802016]
The unique structure of POM microspheres PW9-Si-APBA allows for selective adsorption of phosphoproteins and glycoproteins in biological samples, showing promising potential for practical applications.
Lacunary polyoxometalate (POM), [PW9O34](9-), grafts with a boronic acid group attached via an organosilane bridge assemble into microspheres, PW9-Si-APBA. The oxygen-rich and hydrophilic surface of POM facilitates the binding of phosphate groups in phosphoproteins and glycans in glycoproteins. While the metal-oxo in POM provides pi-pi interactions with the phosphate groups of phosphoproteins, the boronic acid group specifically binds to glycoproteins via the cis-diols of glycans. Therefore, these multi-driving forces ensure the selective adsorption of phosphoproteins and glycoproteins by PW9-Si-APBA microspheres in biological sample matrixes, even in the presence of very high protein abundance, i.e., BSA, at mass ratio of beta-ca/IgG/OVA/BSA = 1 : 1 : 1 : 200.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据