期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 17, 期 5, 页码 1277-1288出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.56657
关键词
-
资金
- National Science Foundation of China [81772345]
- Health Commission of Hubei Province
The upregulation of miR-4485 in COVID-19 patients negatively regulates osteogenic differentiation, potentially targeting Toll-like receptor 4 (TLR-4) and suppressing osteoblastic differentiation in vitro. These findings suggest a promising target for promoting fracture healing and treating osteoporosis in COVID-19 patients.
The angiotensin-converting enzyme 2 (ACE2) receptor has been identified as the cell entry point for SARS-CoV-2. Although ACE2 receptors are present in the bone marrow, the effects of SARS-CoV-2 on the biological activity of bone tissue have not yet been elucidated. In the present study we sought to investigate the impact of SARS-CoV-2 on osteoblastic activity in the context of fracture healing. MicroRNA-4485 (miR-4485), which we found to be upregulated in COVID-19 patients, negatively regulates osteogenic differentiation. We demonstrate this effect both in vitro and in vivo. Moreover, we identified the toll-like receptor 4 (TLR-4) as the potential target gene of miR-4485, and showed that reduction of TLR-4 induced by miR-4485 suppresses osteoblastic differentiation in vitro. Taken together, our findings highlight that up-regulation of miR-4485 is responsible for the suppression of osteogenic differentiation in COVID-19 patients, and TLR-4 is the potential target through which miR-4485 acts, providing a promising target for pro-fracture-healing and anti-osteoporosis therapy in COVID-19 patients.
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