期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 28, 期 5, 页码 754-766出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2017.1312293
关键词
Citrinin; monoclonal antibody; ic-ELISA; lateral-flow ICA strip; cereal
资金
- National Key R D Program [2016YFD0501208, 2016YFF0202300]
A sensitive and specific anti-citrinin (anti-CIT) monoclonal antibody 1F2 was obtained following immunization and cell fusion. An indirect competitive enzyme-linked immunosorbent assay was developed with a 50% inhibitory concentration of 0.761ng/mL and a limit of detection of 0.089ng/mL. The recovery rates for CIT-spiked cereals (maize, wheat, and rice) ranged from 112% to 123%. A lateral-flow immunochromatographic assay was developed for both semi-quantitative and quantitative detection. With CIT-spiked cereals, the visual limit of detection was 8ng/g and the cut-off value was 40ng/g (semi-quantitative analysis with naked-eye detection). Using a strip scan reader, the calculated limit of detection was 1.28-1.8ng/g for different CIT-spiked cereals. The recovery rates ranged from 110% to 127%. Therefore, both methods were effective for CIT detection and suitable for on-site detection and rapid screening of samples.
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