Maternal undernutrition during pregnancy and lactation increases transcription factors, ETV5 and GDNF, and alters regulation of apoptosis and heat shock proteins in the testis of adult offspring in the rat

We tested whether changes in Sertoli cell transcription factors and germ cell heat shock proteins (HSPs) are linked to the effects of maternal undernutrition on male offspring fertility. Rats were fed ad libitum with a standard diet (CONTROL) throughout pregnancy and lactation or with 50% of CONTROL intake throughout pregnancy (UNP) or lactation (UNL) or both periods (UNPL). After postnatal Day 21, 10 male pups per group were fed a standard diet ad libitum until postnatal Day 160 when testes were processed for histological, mRNA and immunohistochemical analyses. Compared with CONTROL: caspase-3 was increased inUNP and UNPL (P = 0.001); Bax was increased inUNL (P = 0.002); Bcl-2 (P < 0.0001) was increased in all underfed groups; glial cell line-derived neurotrophic factor (P = 0.002) was increased in UNP and UNL; E twenty-six transformation variant gene 5 and HSP70 were increased, and HSP90 was diminished in all underfed groups (P < 0.0001). It appears that maternal undernutrition during pregnancy and lactation disrupts the balance between proliferation and apoptosis in germ cells, increasing germ cell production and perhaps exceeding the support capacity of the Sertoli cells. Moreover, fertility could be further compromised by changes in meiosis and spermiogenesis mediated by germ cell HSP90 and HSP70.

Automated summary

The study showed that maternal undernutrition during pregnancy and lactation may disrupt the balance between proliferation and apoptosis in germ cells, leading to increased germ cell production and potentially exceeding the support capacity of Sertoli cells. Furthermore, changes in meiosis and spermiogenesis mediated by germ cell HSP90 and HSP70 could further compromise fertility.