Deoxynivalenol and zearalenone in unprocessed cereals and soybean from different cultivation regions in Croatia

The aim of this study was to investigate the occurrence of deoxynivalenol (DON) and zearalenone (ZEN) in unprocessed cereals and soybean sampled in 2014 and 2015 from different fields located in Croatian regions. A total of 306 samples were analysed for DON and 415 samples for ZEN concentrations using quantitative ELISA methods. In 2014, DON and ZEN were determined in all samples in the mean concentrations of 1,461 +/- 2,265 mu g/kg and 656 +/- 853 mu g/kg, respectively, while in 2015 these means were 2,687 +/- 2,731 mu g/kg and 1,140 +/- 1,630 mu g/kg, respectively. The cultivation year significantly (p<0.05) influenced mycotoxin concentrations, whereas the influence of cultivation region was seen with ZEN for all cereals except soybean, and not seen with DON at all. A higher contamination determined during 2015 could be explained by high to extreme humidity evidenced in the period of cereals' growth and harvesting.