AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled Escherichia coli

A chemical fingerprint of the Escherichia coli cell surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight Secondary Ion Mass Spectroscopy (ToF-SIMS) Tandem MS for unambiguous molecular identification of co-localized species.

Automated summary

This study utilized gold nanoparticles and two mass spectroscopy techniques to obtain a chemical fingerprint of the Escherichia coli cell surface.