期刊
CHEMICAL SCIENCE
卷 12, 期 20, 页码 7151-7157出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1sc00515d
关键词
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资金
- Singapore Ministry of Education [MOE2015-T2-1-092, MOE2012-T3-1-001]
- National Research Foundation Investigatorship [NRF-NRFI2017-09]
- Nanyang Technological University
- European Research Council under the European Union's Seventh Framework Program (ERC) [616551]
- European Research Council (ERC) [616551] Funding Source: European Research Council (ERC)
This study reported the first observation of extremely long-lived K+ and NH4+ ions at the center of a left-handed G-quadruplex DNA. It was found that a single-base mutation could affect this long-lived behavior of the central cation.
Cations are critical for the folding and assembly of nucleic acids. In G-quadruplex structures, cations can bind between stacked G-tetrads and coordinate with negatively charged guanine carbonyl oxygens. They usually exchange between binding sites and with the bulk in solution with time constants ranging from sub-millisecond to seconds. Here we report the first observation of extremely long-lived K+ and NH4+ ions, with an exchange time constant on the order of an hour, when coordinated at the center of a left-handed G-quadruplex DNA. A single-base mutation, that switched one half of the structure from left- to right-handed conformation resulting in a right-left hybrid G-quadruplex, was shown to remove this long-lived behaviour of the central cation.
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