期刊
LAB ON A CHIP
卷 21, 期 8, 页码 1527-1539出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc01194k
关键词
-
类别
资金
- NEI NIH HHS [R01 EY019101] Funding Source: Medline
A three-dimensional skin-on-chip model was developed to study the migration of human T lymphocytes in inflammatory microenvironments. Results showed that T cell migration can be inhibited by engineered CCL20 locked dimer and S1P background, while inflammatory cytokine stimulated epithelial cells can induce T cell transmigration in the model.
A microfluidics-based three-dimensional skin-on-chip (SoC) model is developed in this study to enable quantitative studies of transendothelial and transepithelial migration of human T lymphocytes in mimicked skin inflammatory microenvironments and to test new drug candidates. The keys results include 1) CCL20-dependent T cell transmigration is significantly inhibited by an engineered CCL20 locked dimer (CCL20LD), supporting the potential immunotherapeutic use of CCL20LD for treating skin diseases such as psoriasis; 2) transepithelial migration of T cells in response to a CXCL12 gradient mimicking T cell egress from the skin is significantly reduced by a sphingosine-1-phosphate (S1P) background, suggesting the role of S1P for T cell retention in inflamed skin tissues; and 3) T cell transmigration is induced by inflammatory cytokine stimulated epithelial cells in the SoC model. Collectively, the developed SoC model recreates a dynamic multi-cellular micro-environment that enables quantitative studies of T cell transmigration at a single cell level in response to physiological cutaneous inflammatory mediators and potential drugs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据