Biocompatibility of gold nanoparticles: In-vitro and In-vivo study

Gold nanoparticles have unique characters such as chemical, physical, and optical properties. It have used in biomedical, pharmaceutical, industrial, and other sciences. Thus, for their safe and effective applications, much responsiveness has been given to the side effect and toxicity of gold nanoparticles in biological and medical systems. In this study, we investigated the toxicity of Gold nanoparticles (GNPs) using In-viro and In-vivo study. MIT assay was used to investigate cytotoxic activity of GNPs against Rate embryonic fibroblast (REF) cell line. While, In-vivo model the GNPs were intraperitoneal injected in mice at concertation 100 mu g/Kg. Then, mice body weight and histopathological changes were studied after 4 weeks of injection. GNPs were characterized and confirmed using UV-spectrum assay. Cytotoxicity of GNPs against REF cells showed no toxic effect and no morphological changes in REF cells after treated with GNPs at concentration 1, 5, and 10 mu g/ml. While, histopathological results showed no changes were addressed. The findings demonstrated that the GNPs were biocompatible materials In-vitro and In-vivo. (C) 2021 Elsevier Ltd. All rights reserved.

Automated summary

The study examined the toxicity of gold nanoparticles in both in-vitro and in-vivo settings. Results indicated that GNPs exhibited no toxic effects on REF cells and did not cause significant pathological changes in mice after being injected intraperitoneally.