期刊
BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
卷 14, 期 1, 页码 129-137出版社
SOC SCIENCE & NATURE
DOI: 10.21786/bbrc/14.1/18
关键词
BIOSURFACTANT; PSEUDOMONAS BALEARICA; CONTAMINATION; DEGRADATION; OIL HYDROLYSIS
This study investigated the biosurfactant produced by P. balearica isolated from oil-contaminated sea water to determine optimal production conditions. Olive oil and urea were found to be the best carbon and nitrogen sources, with pH 7, inoculum size 2%, incubation temperature 30℃, and 312 hours of incubation period yielding the highest biosurfactant production. The biosurfactant reduced water surface tension from 72 to 34 mN/m, demonstrating potential for use in oil hydrolysis.
The biosurfactant produced by Pseudomonas balearica isolated from oil-contaminated sea water was studied in the present research. A range of different growth conditions, such as temperature, pH, incubation period, inoculum size and different carbon/nitrogen sources and different C/N ratios were investigated to determine the optimum conditions for maximum production of a biosurfactant by the selected bacterial strain using a mineral salt medium. The best carbon andnitrogen sources were olive oil and urea, giving biosurfactant yields of 6.23 +/- 0.06 and 6.33 +/- 0.10 mg/ml, respectively. The maximum rhamnolipid production was 6.40 +/- 0.14 mg/ml at C/N (olive oil/ urea) of 30. The highest biosurfactant yield was at pH 7 (6.37 +/- 0.06 mg/ml), with inoculum size 2% (6.29 +/- 0.16 mg/ml), and incubation temperature 30 degrees C (6.18 +/- 0.14 mg/ml) and after a 312 hrs incubation period (6.30 +/- 0.09). The previous investigated nutritional and environmental factors showed the highest emulsification activity. The produced rhamnolipid biosurfactant reduced the surface tension of water from 72 to 34 mN/m. In conclusion, the findings herein demonstrated that the rhamnolipid biosurfactant production by P. balearica can be used during oil hydrolysis.
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