期刊
CHEMICAL COMMUNICATIONS
卷 57, 期 30, 页码 3672-3675出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc00594d
关键词
-
The research demonstrates the conversion of a dapoxyl DNA aptamer into a sensor generating fluorescence signal at different wavelengths for detecting drug-resistant tuberculosis gene fragments. The promiscuity of the aptamer in binding to fluorogenic dyes has been discovered, laying the foundation for its application as a versatile sensing platform.
Light-up DNA aptamers are promising label-free signal-transducers for biosensing applications due to their high chemical stability and low synthetic cost. Herein, we demonstrate that a dapoxyl DNA aptamer DAP-10-42 can be converted into a sensor generating a fluorescence signal at different wavelengths in the range of 500-660 nm depending on the dye that is present. This results from the discovered promiscuity of DAP-10-42 in binding fluorogenic dyes including arylmethane dyes. We have designed a split DAP-10-42 aptasensor for the detection of a katG gene fragment from Mycobacterium tuberculosis with a point mutation causing isoniazid resistance. Efficient interrogation of the gene fragment after nucleic acid sequence-based amplification (NASBA) is achieved directly in a protein-containing NASBA sample. This report lays a foundation for the application of the DAP-10-42 aptamer as a versatile sensing platform.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据