4.5 Article

A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells

期刊

PLANT SIGNALING & BEHAVIOR
卷 16, 期 9, 页码 -

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/15592324.2021.1924977

关键词

Ammonium toxicity; endomembrane trafficking; macroautophagy; microautophagy; endocytosis; vacuole morphology; cell elongation

资金

  1. Fondo para la Investigacion Cientifica y Tecnologica [FONCyT PICT-2017-2863]
  2. Ministry of Education, Culture, Sports, Science and Technology, Program for Strategic Research Foundation at Private Universities [S1411023]
  3. Institute of Science and Technology, Meiji University
  4. Meiji University [MU-RMG 2019-11]
  5. Proyecto INTA [2019-PD-E6-I116-001]
  6. [19H05713]

向作者/读者索取更多资源

Ammonium stress affects plant physiology in multiple ways and involves complex mechanisms. Root growth inhibition and cell elongation are key targets of NH4+ stress. Autophagy plays a role in plants' response to NH4+, affecting energy balance and vacuole development.
Ammonium (NH4+) stress has multiple effects on plant physiology, therefore, plant responses are complex, and multiple mechanisms are involved in NH4+ sensitivity and tolerance in plants. Root growth inhibition is an important quantitative readout of the effects of NH4+ stress on plant physiology, and cell elongation appear as the principal growth inhibition target. We recently proposed autophagy as a relevant physiological mechanisms underlying NH4+ sensitivity response in Arabidopsis. In a brief overview, the impaired macro-autophagic flux observed under NH4+ stress conditions has a detrimental impact on the cellular energetic balance, and therefore on the energy-demanding plant growth. In contrast to its inhibitory effect on the autophagosomes flux to vacuole, NH4+ toxicity induced a micro-autophagy-like process. Consistent with the reduced membrane flux to the vacuole related to macro-autophagy inhibition and the increased tonoplast degradation due to enhanced micro-autophagy, the vacuoles of the root cells of the NH4+-stressed plants showed lower tonoplast content and a decreased perimeter/area ratio. As the endosome-to-vacuole trafficking is another important process that contributes to membrane flux toward the vacuole, we evaluated the effects of NH4+ stress on this process. This allows us to propose that autophagy could contribute to vacuole development as well as possible avenues to follow for future studies.

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