Fe(iii)-complex mediated bacterial cell surface immobilization of eGFP and enzymes†

We report a facile and reversible method to immobilize a broad range of His(6)-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. A His(6)-tagged eGFP and four His(6)-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His(6)-tagged HRP.

Automated summary

This study presents a facile and reversible method to immobilize a wide range of His(6)-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. Successfully, an eGFP and four enzymes with His(6)-tag were immobilized on the cell surface. Moreover, a hydrogel sheath around E. coli cells was generated by immobilizing the His(6)-tagged HRP.