期刊
CHINESE JOURNAL OF NATURAL MEDICINES
卷 19, 期 4, 页码 295-304出版社
CHINESE JOURNAL NATURAL MEDICINES
DOI: 10.1016/S1875-5364(21)60030-4
关键词
High performance thin-layer chromatography; Mass spectrometry QDa; Ganoderma; Authentication
资金
- National Key R&D Program of China [2019YFC1711000, 2018YFC1707900]
- National Natural Science Foundation of China [8200140730]
- Qi-Huang Scholar of National Traditional Chinese Medicine Leading Talents Support Program (2018)
A simple and practical HPTLC-QDa method was developed for the robust authentication of Ganoderma lucidum and its confused species, allowing for efficient identification based on characteristic mass spectra.
In this study, a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa (HPTLC-QDa) method for robust authentication of Ganoderma lucidum, a popular and valuable herbal medicine, has been developed. This method is simple and practical, which allows direct generation of characteristic mass spectra from the HPTLC plates automatically with the application of in situ solvent desorption interface. The HPTLC silica gel plates were developed with toluene-ethyl formate-formic acid (5 : 5 : 0.2, V/V) and all bands were transferred to QDa system directly in situ using 80% methanol with 0.1% formic acid as desorption solvent. The acquired HPTLC-QDa spectra showed that luminous yellow band b3, containing ganoderic acid B/G/H and ganodeneric acid B, the major active components of Ganoderma, could be found only in G. lucidum and G. lucidum (Antler-shaped), but not in G. sinense and G. applanatum. Moreover, bands b13 and b14 with m/z 475/477 and m/z 475/491/495, respectively, could be detected in G. lucidum (Antler-shaped), but not in G. lucidum, thus allowing simple and robust authentication of G. lucidum with confused species. This method is proved to be simple, practical and reproducible, which can be extended to analyze other herbal medicines.
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