期刊
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
卷 18, 期 11, 页码 2285-2293出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijms.56198
关键词
Flos Lonicerae Japonicae water extract (FLJWE); pseudorabies virus (PRV); antiviral inflammatory; heme oxygenase-1 (HO-1)
资金
- Ministry of Health and Welfare, Executive Yuan, Taiwan (ROC) [CCMP102-RD-004]
- Chung Shan Medical University [NCHU-CSMU-10708, NCHU-CSMU10613]
- Ministry of Science and Technology, Taiwan [MOST-105-2320-B-040-021, MOST-106-2320-B-040-023-MY3]
The study showed that the Flos Lonicerae Japonicae water extract (FLJWE) and luteolin effectively reduced inflammation in PRV-infected cells by inhibiting NF-kappa B pathway and inducing HO-1 expression, making them potential antiviral agents.
This study examined the effect of the Flos Lonicerae Japonicae water extract (FLJWE), chlorogenic acid, and luteolin on pseudorabies virus (PRV)-induced inflammation in RAW264.7 cells and elucidated related molecular mechanisms. The results revealed that FLJWE and luteolin, but not chlorogenic acid, inhibited the production of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in PRV-infected RAW 264.7 cells. We found that the FLJWE and luteolin suppressed nuclear factor (NF)-kappa B activation by inhibiting the phosphorylation of signal transducer and activator of transcription 1 and 3 (STAT1 and STAT3, respectively). Moreover, the FLJWE significantly upregulated the expression of pNrf2 and its downstream target gene heme oxygenase-1 (HO-1). Our data indicated that FLJWE and luteolin reduced the expression of proinflammatory mediators and inflammatory cytokines, such as COX-2 and iNOS, through the suppression of the JAK/STAT1/3-dependent NF-kappa B pathway and the induction of HO-1 expression in PRV-infected RAW264.7 cells. The findings indicate that the FLJWE can be used as a potential antiviral agent.
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