期刊
FISH & SHELLFISH IMMUNOLOGY
卷 60, 期 -, 页码 318-325出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.10.011
关键词
Clonorchis sinensis; Bacillus subtilis spore; Enolase; Oral administration; Grass carp (Ctenopharyngodon idella)
资金
- Science and Technology Planning Project of Guangdong Province [2013B010404010, 2014B020203001]
- Innovative Research Teams Project of South Wisdom Valley, Shunde, Guangdong province [2013CXTD03]
- Fundamental Research Funds for the Central Universities of China [3161036]
- Young Teachers' Cultivation Project of Basic Scientific Research Service Fee in Colleges and Universities [16ykpy06]
Clonorchiasis, caused by the consumption of raw or undercooked freshwater fish containing infective metacercariae of Clonorchis sinensisis (Csinensis), remains a common public health problem. New effective prevention strategies are still urgent to control this food-borne infectious disease. The previous studies suggested Bacillus subtilis (B. subtilis) spores was an ideal vaccines delivery system, and the C.sinensis enolase (CsENO) was a potential vaccine candidate against clonorchiasis. In the current study, we detected CsENO-specific IgM levels by ELISA in sera, intestinal mucus and skin mucus in grass carps (Ctenopharyngodon idella) through oral administration with B. subtilis spores surface expressing CsENO. In addition, immune-related genes expression was also measured by qRT-PCR. Grass carps orally treated with B. subtilis spores or normal forages were used as controls. The results of ELISA manifested that specific IgM levels of grass carps in CsENO group in sera, intestine mucus and skin mucus almost significantly increased from week 4 post the first oral administration when compared to the two control groups. The levels of specific IgM reached its peak in intestine mucus firstly, then in sera, and last in skin mucus. qRT-PCR results showed that 5 immune-related genes expression had different degree of rising trend in CsENO group when compared to the two control groups. Our study demonstrated that orally administrated with B. subtilis spores expressing CsENO induced innate and adaptive immunity, systemic and local mucosal immunity, and humoral and cellular immunity. Our work may pave the way to clarify the exact mechanisms of protective efficacy elicited by B. subtilis spores expressing CsENO and provide new ideas for vaccine development against C. sinensis infection. (C) 2016 Elsevier Ltd. All rights reserved.
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