Action of miR-133b Targeting Kinesin Family Member 15 on Astrocyte Proliferation and Apoptosis

To determine the effect of miR-133b targeting Kif15 on astrocyte proliferation and apoptosis and its mechanism, miR-133b mimics were transfected to astrocytes. qRT-PCR was applied to test miR-133b levels to verify the transfection effect, and MTT was applied to test the action of miR-133b overexpression on cellular proliferation activity. Flow cytometry was applied to test the action of miR-133b overexpression on the apoptosis rate. Western blot was adopted to detect miR-133b overexpression on cellular recombinant kinesin family member 15 (Kif15), Bcl2, and Bax protein. starBase online software forecasts exhibited that 3'UTR of Kif15 have miR-133b binding sites. The targeting association between miR-133b and Kif15 was verified using a double-luciferase reporter gene assay. By comparison with the miR-con group, there was a reduction in astrocyte absorption values and Bcl2 protein expression in the miR-133b group (P < 0.05) and an increase in the apoptosis rate and Bax protein expression level (P < 0.05). Kif15 was negatively regulated by miR-133b in astrocytes. The overexpression of Kif15 reversed the action of miR-133b on astrocyte proliferation and apoptosis. Overexpression of miR-133b probably inhibited the proliferative activity of astrocytes and induced their apoptosis by targeting down- regulated Kif15 expression.

Automated summary

The research revealed that miR-133b plays a regulatory role in astrocyte proliferation and apoptosis by targeting down-regulated expression of Kif15 protein. Overexpression of miR-133b led to decreased cellular proliferation and increased apoptosis rates, possibly through down-regulating Kif15 expression.