期刊
FISH & SHELLFISH IMMUNOLOGY
卷 60, 期 -, 页码 326-333出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.12.003
关键词
Bacillus subtilis HAINUP40; Tilapia (Oreochromis niloticus); Growth; Digestive enzyme activity; Innate immunity; Protection
资金
- National Natural Science Foundation of China [31560725, 31260644, 41266004]
- Public Science and Technology Research Funds Projects of Ocean [201405020-4]
- Agricultural science and technology achievement transformation project [2014GB2E200127]
- Research Fund for the Doctoral Program of Higher Education [20124601110006]
- Hainan Special Funding for Invigorating the Marine Economy through Science and Technology [2015XH02]
- Youth Foundation of Hainan University [qnjj1206]
The probiotic properties of Bacillus subtilis HAINUP40 isolated from the aquatic environment, and the effects of dietary administration of B. subtilis HAINUP40 on the growth performance, intestinal probiotic recovery, digestive enzyme activities, innate immunity and disease resistance of tilapia (Oreochromis niloticus) were evaluated. The probiotic properties investigated include tolerance to simulated gastrointestinal stress, auto-aggregation, cell surface hydrophobicity and extracellular enzyme production. The cell number of B. subtilis changed little after 4 h in simulated gastric fluid at pH = 2.0, 3.0, 4.0 and simulated intestinal fluid at pH = 6.8.B.subtilis HAINUP40 revealed strong auto-aggregation property (34.6-87.0%) after 24 h incubation period. It exhibited significant cell surface hydrophobicity in xylene (28.8%) and chloroform (41.3%) and produced extracellular proteases and amylase. After tilapia (mean weight = 95 +/- 8 g) were fed with a diet containing 10(8) cfu/g B. subtilis HAINUP40, their final body weight, percent weight gain (PWG), specific growth rate (SGR), total antioxidant capacity (T-AOC) and serum superoxide dismutase (SOD) increased significantly (p < 0.05) after 8 weeks; feed conversion rate (FCR) is significantly lower (p < 0.05) after 8 weeks; the protease and amylase activity in the digestive tract increased significantly (p < 0.05) after 4 and 8 weeks; and respiratory bursts and serum lysozyme activity increased significantly (p < 0.05) after 2 weeks. Moreover, being challenged with pathogenic Streptococcus agalactiae for 2 weeks, the relative percent survival (RPS%) is 52.94%. The results of this study strongly suggest that dietary supplement of B. subtilis HAINUP40 can effectively enhances the growth performance, immune response, and disease resistance of Nile tilapia. (C) 2016 Elsevier Ltd. All rights reserved.
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