期刊
FISH & SHELLFISH IMMUNOLOGY
卷 62, 期 -, 页码 195-201出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2017.01.015
关键词
Lactobacillus plantarum; Litopenaeus vannamei; Growth performance; Immune gene; Low salinity stress
资金
- National Science & Technology Pillar Program during the 12th Five-year Plan Period [2011BAD13B10]
- Guangdong Provincial Special Fund for Marine Fisheries Technology [A201501B15]
- Guangdong Provincial Marine Economy Innovation Development Demonstration Project [GD2013-B03-005]
- Natural Science Foundation of Guangdong Province [2015A030310393]
- Special Scientific Research Funds for Central Non-profit Institutes, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences [2016TS07]
A 45-day feeding trial followed by an acute stress test of low salinity was done to evaluate effects of Lactobacillus plantarum on growth performance and anti-stress capability of white shrimp (Litopenaeus vannamei). Shrimp were randomly allocated in 15 tanks (100 shrimp per tank) and divided into 5 treatments with 3 replicates. Triplicate tanks were fed with a control diet or diets containing different treatments of L. plantarum (fermentation supernatant (FS), live bacteria (LB), dead bacteria (DB) and cell free extract (CE) of L. plantarum) as treatment groups. Growth performance including weight gain (WGR), feed conversion ratio (FCR) and specific growth rate (SGR) were determined after feeding 45 days. Anti stress capacity was evaluated by determining the gene expression of ProPO, SOD and Lys in gut of shrimp at the end of feeding trial and again at 96 h post-stress test. Results indicated that supplementation of L. plantarum into diet had significantly improved growth performance of L. vannamei. On the other hand, L. plantarum supplementation had no significant effects on the gene expression of SOD and Lys in gut of shrimp cultured under normal condition for 45 days. Supplementation of L plantarum had increased survivability of L. vannamei having higher survival rates compared to the control group. However, statistical analysis showed no significant difference between the control group and treatments. Compared with the control group, supplementation of L. plantarum significantly improved the resistance of L. vannamei against the stress of acute low salinity, as indicated by higher survival rate as well as higher transcript levels of ProPo, SOD and Lys gene. Our findings suggested that L. plantarum, especially cell-free extract of L. plantarum has improved the anti-stress capacity of L. vannamei and could serve as a potential feed additive that helps shrimp to overcome environmental stresses. (C) 2017 Elsevier Ltd. All rights reserved.
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