期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 85, 期 4, 页码 972-980出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbaa112
关键词
ammonia; cell surface engineering; Saccharomyces cerevisiae; l-amino acid oxidase; food processing waste
类别
资金
- Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) [JPMJCR16G2]
Producing ammonia outside of yeast cells through cell surface engineering is an efficient approach to avoid growth deficiencies caused by ammonia assimilation gene knockout. Displaying HcLAAO on yeast cell surfaces successfully produced ammonia with a conversion efficiency higher than previous studies. This study demonstrates the promising strategy of utilizing food processing wastes for ammonia production.
Ammonia is critical for agricultural and chemical industries. The extracellular production of ammonia by yeast (Saccharomyces cerevisiae) using cell surface engineering can be efficient approach because yeast can avoid growth deficiencies caused by knockout of genes for ammonia assimilation. In this study, we produced ammonia outside the yeast cells by displaying an l-amino acid oxidase with a wide substrate specificity derived from Hebeloma cylindrosporum (HcLAAO) on yeast cell surfaces. The HcLAAO-displaying yeast successfully produced 12.6 mm ammonia from a mixture of 20 proteinogenic amino acids (the theoretical conversion efficiency was 63%). We also succeeded in producing ammonia from a food processing waste, soybean residues (okara) derived from tofu production. The conversion efficiency was 88.1%, a higher yield than reported in previous studies. Our study demonstrates that ammonia production outside of yeast cells is a promising strategy to utilize food processing wastes.
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