期刊
CLINICAL LABORATORY
卷 67, 期 2, 页码 425-427出版社
CLIN LAB PUBL
DOI: 10.7754/Clin.Lab.2020.200731
关键词
2019-nCoV; virus preservation solution; ribonucleic acid detection; RT-PCR
资金
- Sichuan Medical Scientific Research Foundation [Q18025]
The accuracy of nucleic acid detection for SARS-CoV-2 can be impacted by various factors, including the use of unmatched virus preservation solution which may lead to incorrect clinical diagnosis.
Background and Methods: 2019 Corona Virus Disease (COVID-19) caused by SARS-CoV-2 is still pandemic now. RT-qPCR detection was the most common method for the diagnosis of SARS-CoV-2 infection, facilitated by amounts of nucleic acid testing kits. However, the accuracy of nucleic acid detection is affected by various factors such as specimen collection, specimen preparation, reagents deficiency, and personnel quality. Results: In this study, we found that unmatched virus preservation solution will inhibit N gene and OFR-1ab gene (two independent genes of SARS-CoV-2) amplification in one-step detection reagent. Conclusions: Despite just being a particular phenomenon we found in our work to fight 2019-nCoV, we concluded that unmatched virus preservation solution may have an inhibitory effect on SARS-CoV-2 nucleic acid detection which may lead to incorrect clinical diagnosis.
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